PURIFICATION AND PROPERTIES OF A β-HYDROXYSTEROID DEHYDROGENASE

Purification and properties of the soluble 17 beta-hydroxysteroid dehydrogenase of rabbit uterus.

17 beta-Hydroxysteroid dehydrogenase activity towards estradiol-17 beta has been demonstrated in the 105,000 x g supernatant of rabbit uterus. Hydroxylapatite chromatography of the enzyme activity isolated by ammonium sulfate precipitation, gel filtration and DEAE-cellulose chromatography yielded a single 17 beta-hydroxysteroid dehydrogenase activity. Further purification of the enzyme preparat...

Purification and properties of milk xanthine dehydrogenase.

Milk xanthine oxidase (XO) has been prepared in a dehydrogenase form (XDH) by purifying the enzyme in the presence of 2.5 mM dithiothreitol. Unlike XO, which reacts rapidly only with oxygen and not with NAD, the XDH form of the enzyme reacts rapidly with NAD. XDH has a turnover number for the NAD-dependent conversion of xanthine to urate of 380 mol/min/mol at pH 7.5, 25 degrees C, with a Km = <...

Purification of a 17beta-hydroxysteroid dehydrogenase of human placenta and studies on its transhydrogenase function.

This paper describes methods for the extensive purification of a soluble 17@-hydroxysteroid dehydrogenasel of human placenta. This single enzyme appears to be responsible for the entire 17/3estradiol-mediated transfer of hydrogen between pyridine nucleotides in soluble extracts of this tissue. Experiments which were reported in 1958 (2, 3) showed that soluble enzyme preparations derived from hu...

Properties of an antiserum against Streptomyces hydrogenans 20beta-hydroxysteroid dehydrogenase.

Antiserum against crystallized 20beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was used for different immunodiffusion and immunoprecipitation tests to quantify the bacterial enzyme in cell-free supernatants of the microorganism. After immunoprecipitation and gel electrophoresis the molecular weight of the subunits of 20beta-hydroxysteroid dehydrogenase was calculated to be 27 ...

Brain aldehyde dehydrogenase. Localization, purification, and properties.